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ImageJ

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Here we have a comparative analysis of ImageJ and Pixcavator 2.4.

Tasks and features

ImageJ

Pixcavator

Analysis of the gray scale image after binarization

Yes

Yes

Computation of binary characteristics of objects/particles

Yes

(A specific binarization has to be found first by thresholding or another method.)

Yes

(The characteristics are computed for all possible thresholds.)

size/area

Yes

Yes

circularity/roundness

Yes

Yes

centroid

Yes

Yes

perimeter

Yes

Yes

bounding rectangle

Yes

No

(Useless for such applications as microscopy where the results should be independent of orientation)

Analysis of the gray scale image without prior binarization

Limited

Yes

Detection of objects as max/min of the gray scale

Yes

Yes

Filtering detected objects (in order to deal with noise etc)

Yes

(with respect to contrast only)

Yes

(with respect area, contrast, roundness, and saliency)

Counting objects/particles

Yes

Yes

Image segmentation method

Watershed - for either max or min but not both (dark or light objects but not both)

Topology (both dark and light objects)

Computation of gray scale characteristics of objects

No

Yes

contrast

No

Yes

center of mass

No

Yes

saliency/mass

No

Yes

average contrast

No

Yes

Automatic analysis

Yes

Yes

Semi-automatic mode

No

Yes

(based on objects found for all possible thresholds)

Manual mode

No

Yes

(full control over found objects)

User interface

Hundreds of commands in drop down menus

4 sliders, 7 buttons

User experience (mine)

“Wrong image format!”

“Threshold first!”

“Results unsatisfactory? Start over!”

Move sliders, click buttons

Screenshots

Platforms
Windows, Mac, Linux
Windows only (cellAnalyst web application is available)
Price
Free
$150 (free trial)


See also other Image analysis software. Download the free Pixcavator Student Edition here.
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